Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PSMB10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1 |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.1) Bailey, S.D., et al. Diabetes Care (2010) In press : |
| Immunogen | This PSMB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human PSMB10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMB10 |
|---|---|
| Alternative Names | Proteasome subunit beta type-10 Low molecular mass protein 10 Macropain subunit MECl-1 Multicatalytic endopeptidase complex subunit MECl-1 Proteasome MECl-1 Proteasome subunit beta-2i PSMB10 LMP10 MECL1 |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
