Application
Western Blot at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 ug per lane.Application
PSMB11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PSMB11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proteasome subunit beta type-11, Proteasome subunit beta-5t, PSMB11 |
| Product description | Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes, complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7, and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM 600306). Three additional subunits, beta-1i (PSMB9; MIM 177045), beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11, or beta-5t, is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al., 2007 [PubMed 17540904]).1) Tomaru, U., et al. Blood 113(21):5186-5191(2009) |
| Immunogen | This PSMB11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human PSMB11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMB11 |
|---|---|
| Alternative Names | Proteasome subunit beta type-11 Proteasome subunit beta-5t PSMB11 |
| Molecular Weight(MW) | 33 kDa |
Application
Western Blot at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 ug per lane.
Application
PSMB11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
