Application
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.| Product Name | PSMD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMD4, AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5 |
| Product description | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD4 encodes one of the non-ATPase subunits of the 19S regulator lid.1) Fujiwara, K., et al., (2004) J. Biol. Chem. 279 (6), 4760-4767. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMD4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PSMD4 antibody can be used for detection of PSMD4 by ELISA at 1:62500. PSMD4 antibody can be used for detection of PSMD4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMD4 |
|---|---|
| Alternative Names | PSMD4 AF ASF S5A AF-1 MCB1 Rpn10 pUB-R5 |
| Molecular Weight(MW) | 41 kDa |
Application
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | PSMD4 antibody can be used for detection of PSMD4 by ELISA at 1:62500. PSMD4 antibody can be used for detection of PSMD4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PSMD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
