Application
Western blot analysis of TM yeast Pst1 protein (50 ng) with Pst1 antibody at 1 μg/mL.| Product Name | Pst1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pst1 Antibody: HPF2, HPF2, YDR055W, D4214, YD9609.09, Cell wall mannoprotein PST1, Haze protective factor 2 |
| Product description | Pst1 Antibody: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.1) Pardo M, Monteoliva L, Pla J, et al. Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast 1999; 15:459-72. |
| Immunogen | Pst1 antibody was raised against a synthetic peptide from the amino terminus of yeast Pst1. The immunogen is located within amino acids 80 - 130 of Pst1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 μg/mL.: |
|
| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PST1 |
|---|---|
| Alternative Names | Pst1 Antibody: HPF2 HPF2 YDR055W D4214 YD9609.09 Cell wall mannoprotein PST1 Haze protective factor 2 |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (79%), Rat: (79%), Human: (79%) |
Application
Western blot analysis of TM yeast Pst1 protein (50 ng) with Pst1 antibody at 1 μg/mL.| Application Notes | Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Pst1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Pst1 Antibody is supplied in PBS containing 0.02% sodium azide. |
