Application
Immunohistochemistry of PTCHD2 in human kidney tissue with PTCHD2 antibody at 2.5 μg/mL.Application
Immunofluorescence of PTCHD2 in human kidney tissue with PTCHD2 antibody at 20 μg/mL.Application
Western blot analysis of PTCHD2 in mouse kidney tissue lysate with PTCHD2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PTCHD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTCHD2 Antibody: DISP3, DISP3, KIAA1337, Patched domain-containing protein 2, Protein dispatched homolog 3 |
| Product description | PTCHD2 Antibody: PTCHD2, also known as DISP3, is the third of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored secreted proteins. Like DISP1 and DISP2, DISP3 has been implicated in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis. It is highly expressed in Purkinje cells, hippocampal neurons, and retinal ganglion cells. Recently, it has been shown that PTCHD2 localizes within the endoplasmic reticulum and colocalizes with cholesterol, and given that its expression is regulated by thyroid hormone (T3), it has been suggested that DISP3 may be a link between thyroid hormone and cholesterol metabolism.1) Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6 |
| Immunogen | PTCHD2 antibody was raised against a 19 amino acid synthetic peptide from near the center of human PTCHD2. The immunogen is located within amino acids 780 - 830 of PTCHD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PTCHD2 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTCHD2 |
|---|---|
| Alternative Names | PTCHD2 Antibody: DISP3 DISP3 KIAA1337 Patched domain-containing protein 2 Protein dispatched homolog 3 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least two isoforms of PTCHD2 are known to exist. This antibody is specific for PTCHD2 and will not recognize the other DISP family of proteins. |
Application
Immunohistochemistry of PTCHD2 in human kidney tissue with PTCHD2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of PTCHD2 in human kidney tissue with PTCHD2 antibody at 20 μg/mL.
Application
Western blot analysis of PTCHD2 in mouse kidney tissue lysate with PTCHD2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | PTCHD2 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PTCHD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PTCHD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
