Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).Application
Western blot analysis of lysed extracts from HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).| Product Name | PTEN (phospho Ser380 / Thr382 / Thr383) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, Mutated in multiple advanced cancers 1, Protein-tyrosine phosphatase PTEN |
| Product description | Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3, 4, 5)P3 > PtdIns(3, 4)P2 > PtdIns3P > Ins(1, 3, 4, 5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.1) Al-Khouri AM, et al. (2005) J Biol Chem. 280 (42): 35195-35202. Torres J, et al. (2001) J Biol Chem. 276 (2): 993-998. Vazquez F, et al. (2000) Mol Cell Biol. 20 (14): 5010-5018. |
| Immunogen | PTEN (Phospho-Ser380/Thr382/Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S (p) -D-T (p) -T (p) -D-S) derived from Human PTEN. |
| Modification | p-Ser380 / Thr382 / Thr383 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTEN |
|---|---|
| Alternative Names | BZS DEC CWS1 GLM2 MHAM TEP1 MMAC1 PTEN1 10q23del CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A Mutated in multiple advanced cancers 1 Protein-tyrosine phosphatase PTEN |
| Molecular Weight(MW) | 54 kDa |
| Tissue Specificity | This antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383. |
| Entrez Gene | 5728 |
|---|---|
| Protein Accession | NP_000305.3 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).
Application
Western blot analysis of lysed extracts from HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
