Application
Immunofluorescence of PTEN in P815 cells with PTEN antibody at 2 μg/mL.Application
Western blot analysis of PTEN in human brain tissue lysate with PTEN antibody at (A) 1 and (B) 2 μg/mL..| Product Name | PTEN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTEN Antibody: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1 |
| Product description | PTEN Antibody: PTEN (phosphatase and tensin homologue deleted on chromosome ten) is a dual-specificity phosphatase (with both protein and lipid phosphatase activity) first identified as a tumor suppressor gene. PTEN indirectly activates the AKT/PI3K pathway, an important signaling pathway for cell growth and proliferation by keeping levels of the second messenger PIP3 low, thereby preventing phosphoinositide-dependent kinase-1 (PDK-1) from phosphorylating and activating AKT. Expression of wild-type PTEN causes growth arrest in many cancer cell lines, but expression of a PTEN protein containing a mutation that blocks its lipid phosphatase activity failed to suppress growth of glioma cell lines suggesting that the tumor suppressive effect of PTEN is mediated solely by its lipid phosphatase activity. Other activities include the inhibition of insulin stimulated MAPK activation by blocking the insulin-receptor substrate (IRS)-1 phosphorylation and assembly of the IRS-1/Grb2/Sos complex.1) Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science1997; 275:1943-7. |
| Immunogen | PTEN antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PTEN. The immunogen is located within the first 50 amino acids of PTEN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| PTEN antibody can be used for the detection of PTEN by Western blot at 1 to 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTEN |
|---|---|
| Alternative Names | PTEN Antibody: BZS DEC CWS1 GLM2 MHAM TEP1 MMAC1 PTEN1 10q23del Phosphatidylinositol 3 4 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Mutated in multiple advanced cancers 1 |
| Molecular Weight(MW) | Predicted: 44, 63 kDa Observed: 46 kDa |
| Sequence Similarities | 3) Cat. No. 17-206 - P815 Cell Slide |
Application
Immunofluorescence of PTEN in P815 cells with PTEN antibody at 2 μg/mL.
Application
Western blot analysis of PTEN in human brain tissue lysate with PTEN antibody at (A) 1 and (B) 2 μg/mL..| Positive Control | 1) Cat. No. 1286 - P815 Cell Lysate |
|---|---|
| Application Notes | PTEN antibody can be used for the detection of PTEN by Western blot at 1 to 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PTEN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PTEN Antibody is supplied in PBS containing 0.02% sodium azide. |
