Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PTGS1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Fluorescent image of Hela cell stained with PTGS1 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTGS1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7unitsApplication
Western blot analysis in A431,K562 cell line lysates (35ug/lane).| Product Name | PTGS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prostaglandin G/H synthase 1, Cyclooxygenase-1, COX-1, Prostaglandin H2 synthase 1, PGH synthase 1, PGHS-1, PHS 1, Prostaglandin-endoperoxide synthase 1, PTGS1, COX1 |
| Product description | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis.1) Helmersson,J., Prostaglandins Leukot. Essent. Fatty Acids 80 (1), 51-56 (2009)Malkowski,M.G., Science 289 (5486), 1933-1937 (2000) |
| Immunogen | This PTGS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human PTGS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PTGS1 |
|---|---|
| Alternative Names | Prostaglandin G/H synthase 1 Cyclooxygenase-1 COX-1 Prostaglandin H2 synthase 1 PGH synthase 1 PGHS-1 PHS 1 Prostaglandin-endoperoxide synthase 1 PTGS1 COX1 |
| Molecular Weight(MW) | 69 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PTGS1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Fluorescent image of Hela cell stained with PTGS1 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTGS1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units
Application
Western blot analysis in A431,K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
