Application
Confocal immunofluorescent analysis of PTK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PTK2 using rabbit polyclonal PTK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).| Product Name | PTK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, PTK2, FAK, FAK1 |
| Product description | PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.1) Calalb,M.B., et.al., Mol. Cell. Biol. 15 (2), 954-963 (1995) Polte,T.R. et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (23), 10678-10682 (1995) Gervais,F.G., et.al., J. Biol. Chem. 273 (27), 17102-17108 (1998) |
| Immunogen | This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-848 amino acids from the C-terminal region of human PTK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | Focal adhesion kinase 1 FADK 1 Focal adhesion kinase-related nonkinase FRNK Protein phosphatase 1 regulatory subunit 71 PPP1R71 Protein-tyrosine kinase 2 p125FAK pp125FAK PTK2 FAK FAK1 |
| Molecular Weight(MW) | 119 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of PTK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PTK2 using rabbit polyclonal PTK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
