PTN is a heparin binding mitogenic protein. It has neurite extension activity.1) Parthymou, A., (2008) Eur. J. Cell Biol. 87 (1), 17-29.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PTN.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PTN antibody can be used for detection of PTN by ELISA at 1:12500. PTN antibody can be used for detection of PTN by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
PTN antibody can be used for detection of PTN by ELISA at 1:12500. PTN antibody can be used for detection of PTN by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PTN antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.