Application
PTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).| Product Name | PTN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Heparin-binding growth-associated molecule, HB-GAM, Heparin-binding neurite outgrowth-promoting factor 1, HBNF-1, Osteoblast-specific factor 1, OSF-1, PTN, HBN |
| Product description | PTN ia a heparin binding mitogenic protein. It has neurite extension activity.1) Li, F., et al. J. Biol. Chem. 285(36):27673-27685(2010) |
| Immunogen | This PTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-154 amino acids from the C-terminal region of human PTN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTN |
|---|---|
| Alternative Names | Pleiotrophin PTN Heparin-binding brain mitogen HBBM Heparin-binding growth factor 8 HBGF-8 Heparin-binding growth-associated molecule HB-GAM Heparin-binding neurite outgrowth-promoting factor 1 HBNF-1 Osteoblast-specific factor 1 OSF-1 PTN HBN |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
PTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
