Application
Immunofluorescence analysis of PTP4A2 Antibody with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of widr cells using PTP4A2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PTP4A2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein tyrosine phosphatase type IVA 2, HU-PP-1, OV-1, PTP(CAAXII), Protein-tyrosine phosphatase 4a2, Protein-tyrosine phosphatase of regenerating liver 2, PRL-2, PTP4A2, PRL2, PTPCAAX2 |
| Product description | PTP4A2 belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity.1) Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007) |
| Immunogen | This PTP4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the Central region of human PTP4A2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTP4A2 |
|---|---|
| Alternative Names | Protein tyrosine phosphatase type IVA 2 HU-PP-1 OV-1 PTP(CAAXII) Protein-tyrosine phosphatase 4a2 Protein-tyrosine phosphatase of regenerating liver 2 PRL-2 PTP4A2 PRL2 PTPCAAX2 |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Immunofluorescence analysis of PTP4A2 Antibody with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of widr cells using PTP4A2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
