Application
Immunohistochemistry of PTPN5 in mouse brain tissue with PTPN5 antibody at 5 μg/ml.Application
Immunofluorescence of PTPN5 in mouse brain tissue with PTPN5 antibody at 20 μg/ml.Application
Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | PTPN5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, PTPSTEP, Striatum-enriched protein-tyrosine phosphatase, STEP |
| Product description | The protein tyrosine phosphatase PTPN5, also known as Striatal enriched phosphatase (STEP), is involved in the regulation of synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors (1). It is expressed in dopaminoceptive neurons of the central nervous system and multiple forms of PTPN5 show differential enrichment in adult brain regions (2). NMDA-mediated activation of PTPN5 is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, PTPN5 is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). PTPN5 may play a role in Alzheimer's disease (1).1) Braithwaite SP, Paul S, Nairn AC, et al. Synaptic plasticity: one STEP at a time. Trends Neurosci. 2006; 29:452-8. |
| Immunogen | PTPN5 antibody was raised against an 18 amino acid peptide near the amino terminus of human PTPN5. The immunogen is located within amino acids 180 - 230 of PTPN5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PTPN5 antibody can be used for detection of PTPN5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPN5 |
|---|---|
| Alternative Names | Tyrosine-protein phosphatase non-receptor type 5 Neural-specific protein-tyrosine phosphatase PTPSTEP Striatum-enriched protein-tyrosine phosphatase STEP |
| Molecular Weight(MW) | Predicted: 62 kDa Observed: 60 kDa |
| Tissue Specificity | PTPN5 antibody is human and mouse reactive. |
Application
Immunohistochemistry of PTPN5 in mouse brain tissue with PTPN5 antibody at 5 μg/ml.
Application
Immunofluorescence of PTPN5 in mouse brain tissue with PTPN5 antibody at 20 μg/ml.
Application
Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PTPN5 antibody can be used for detection of PTPN5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PTPN5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PTPN5 antibody is supplied in PBS containing 0.02% sodium azide. |
