Application
Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Immunofluorescent analysis of U251 cells, using PTTG1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).Application
Western Blot at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PTTG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.1) Li,T.,Genet Genomics 36 (6), 335-342 (2009) |
| Immunogen | This PTTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human PTTG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:500 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PTTG1 |
|---|---|
| Alternative Names | Securin Esp1-associated protein Pituitary tumor-transforming gene 1 protein Tumor-transforming protein 1 hPTTG PTTG1 EAP1 PTTG TUTR1 |
| Molecular Weight(MW) | 22 kDa |
Application
Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Immunofluorescent analysis of U251 cells, using PTTG1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Application
Western Blot at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:500 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
