Application
Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloiApplication
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Product Name | PTTG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Securin-2, Pituitary tumor-transforming gene 2 protein, PTTG2 |
| Product description | 1) Chen, L., et al. Gene 248 (1-2), 41-50 (2000) : |
| Immunogen | This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTTG2 |
|---|---|
| Alternative Names | Securin-2 Pituitary tumor-transforming gene 2 protein PTTG2 |
| Molecular Weight(MW) | 22 kDa |
Application
Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloi
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
