PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.1) Scott, R.E., (2005) J. Cell. Physiol. 204 (1), 99-105.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PUM2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PUM2 antibody can be used for detection of PUM2 by ELISA at 1:62500. PUM2 antibody can be used for detection of PUM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
PUM2 antibody can be used for detection of PUM2 by ELISA at 1:62500. PUM2 antibody can be used for detection of PUM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PUM2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt