Application
Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).| Product Name | PUM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.1) Kusz,K., Mol. Reprod. Dev. 74 (6), 795-799 (2007) |
| Immunogen | This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from human PUM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUM2 |
|---|---|
| Alternative Names | Pumilio homolog 2 Pumilio-2 PUM2 KIAA0235 PUMH2 |
| Molecular Weight(MW) | 114 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
