Application
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | PUMA Antibody [2A8F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.1) Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94. |
| Immunogen | Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BBC3 |
|---|---|
| Alternative Names | PUMA Antibody [2A8F6] : JFY1 PUMA JFY-1 |
| Sequence Similarities | 2) Cat. No. 95-102 - PUMA Recombinant Protein |
Application
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PUMA monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
