Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Product Name | PUS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PUS1, MPUS1, mPus1p, A730013B20Rik |
| Product description | Pus1 converts specific uridines to PSI in a number of tRNA substrates. Pus1 acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Pus1 is involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.1) Zhao, X., et al., (2004) Mol. Cell 15 (4), 549-558. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PUS1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PUS1 antibody can be used for detection of PUS1 by ELISA at 1:312500. PUS1 antibody can be used for detection of PUS1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUS1 |
|---|---|
| Alternative Names | PUS1 MPUS1 mPus1p A730013B20Rik |
| Molecular Weight(MW) | 43 kDa |
| Entrez Gene | 56361 |
|---|---|
| Protein Accession | NP_001020733 |
Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | PUS1 antibody can be used for detection of PUS1 by ELISA at 1:312500. PUS1 antibody can be used for detection of PUS1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PUS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
