Application
PUS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | PUS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.1) Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278(2010) |
| Immunogen | This PUS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human PUS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUS1 |
|---|---|
| Alternative Names | tRNA pseudouridine synthase A mitochondrial tRNA pseudouridine(38-40) synthase tRNA pseudouridylate synthase I tRNA-uridine isomerase I PUS1 |
| Molecular Weight(MW) | 47 kDa |
Application
PUS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
