Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of MKP3 using rabbit polyclonal MKP3-His6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MKP3 gene.| Product Name | R MKP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis1) Muda,M., et al. J. Biol. Chem. 271 (8), 4319-4326 (1996) |
| Immunogen | This Rat MKP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~381 of rat MKP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Dusp6 |
|---|---|
| Alternative Names | Dual specificity protein phosphatase 6 Mitogen-activated protein kinase phosphatase 3 MAP kinase phosphatase 3 MKP-3 Dusp6 Mkp3 |
| Molecular Weight(MW) | 42 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of MKP3 using rabbit polyclonal MKP3-His6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MKP3 gene.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
