Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB8A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of RAB8A Antibody in Hela cell line lysates (35ug/lane)Application
Western Blot at 1:1000 dilution + HCT116 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RAB8A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ras-related protein Rab-8A, Oncogene c-mel, RAB8A, MEL, RAB8 |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. RAB8A is a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.1) Kato,S., et.al., Gene 150 (2), 243-250 (1994) |
| Immunogen | This RAB8A antibody is generated from rabbits immunized with human RAB8A recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB8A |
|---|---|
| Alternative Names | Ras-related protein Rab-8A Oncogene c-mel RAB8A MEL RAB8 |
| Molecular Weight(MW) | 24 kDa |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB8A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of RAB8A Antibody in Hela cell line lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution + HCT116 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
