Application
Immunohistochemistry of RABEX5 in mouse brain tissue with RABEX5 antibody at 5 μg/mL.Application
Immunofluorescence of RABEX5 in mouse brain tissue with RABEX5 antibody at 20 μg/mL.Application
Western blot analysis of RABEX5 in human liver tissue lysate with RABEX5 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RABEX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RABEX5 Antibody: RABEX5 is a guanine nucleotide exchange factor (GEF) that forms a complex with rabaptin-5 and specifically activates Rab5, a small GTPase that plays an essential role in endocytic traffic. RABEX-5 is thought to also interact with another member of the Rab family, Rab22, which targets RABEX5 to early endosomes, allowing it to activate Rab5 and thereby promote Rab5-mediated endosome fusion.1) Horiuchi H, Lippe R, McBride HM, et al. A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function. Cell 1997; 90:1149-59. |
| Immunogen | RABEX5 antibody was raised against a 19 amino acid synthetic peptide near the center terminus of human RABEX5. The immunogen is located within amino acids 430 - 480 of RABEX5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RABEX5 antibody can be used for detection of RABEX5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RABGEF1 |
|---|---|
| Alternative Names | RABEX5 Antibody: RAP1 RABEX5 rabex-5 RAP1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members. |
Application
Immunohistochemistry of RABEX5 in mouse brain tissue with RABEX5 antibody at 5 μg/mL.
Application
Immunofluorescence of RABEX5 in mouse brain tissue with RABEX5 antibody at 20 μg/mL.
Application
Western blot analysis of RABEX5 in human liver tissue lysate with RABEX5 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | RABEX5 antibody can be used for detection of RABEX5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RABEX5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RABEX5 Antibody is supplied in PBS containing 0.02% sodium azide. |
