Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: C6 whole cell lysate Lane 6: H-4-II-E whole cell lysate Lane 7: MOLT-4 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RAD21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.1) McKay M.J.,et al.Genomics 36:305-315(1996). |
| Immunogen | This RAD21 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 501-535 amino acids from human RAD21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD21 |
|---|---|
| Alternative Names | Double-strand-break repair protein rad21 homolog hHR21 Nuclear matrix protein 1 NXP-1 SCC1 homolog RAD21 HR21 KIAA0078 NXP1 |
| Molecular Weight(MW) | 72 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: C6 whole cell lysate Lane 6: H-4-II-E whole cell lysate Lane 7: MOLT-4 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
