Application
Immunohistochemistry staining of RAD52 in prostate tissue using RAD52 Antibody.| Product Name | RAD52 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma). |
| Immunogen | RAD52 antibody was raised against amino acids 122 - 135 of RAD52 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| RAD52 antibody can be used in Western Blot at 1:1000, immunohistochemistry starting at 1:25 - 1:100, and immunoprecipitation starting at 1:50. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD52 |
|---|---|
| Alternative Names | RAD52 RAD52 (S. cerevisiae) homolog RAD52 homolog (S. cerevisiae) Recombination protein RAD52 |
Application
Immunohistochemistry staining of RAD52 in prostate tissue using RAD52 Antibody.| Application Notes | RAD52 antibody can be used in Western Blot at 1:1000, immunohistochemistry starting at 1:25 - 1:100, and immunoprecipitation starting at 1:50. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store RAD52 antibody at -20 ˚C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 ˚C or below. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
