Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti in Hela cell line lysates (35ug/lane)| Product Name | RAD9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.1) Maniwa, Y., et al., Cancer 103(1):126-132 (2005). |
| Immunogen | This RAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human RAD9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD9A |
|---|---|
| Alternative Names | Cell cycle checkpoint control protein RAD9A hRAD9 DNA repair exonuclease rad9 homolog A RAD9A |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
