Application
Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.Application
Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL.| Product Name | RAET1E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Retinoic acid early transcript 1E, NKG2D ligand 4, N2DL-4, NKG2DL42, RL-4, ULBP4, LETAL |
| Product description | The Retinoic acid early transcript 1E (RAET1E) belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3 (1). Like the related protein RAET1G, RAET1E differs from other RAET1 proteins in that they have type I membrane-spanning sequences at their carboxy termini rather than glycosylphosphatidylinositol anchor sequences (2). RAET1E functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses (1,3).1) Radosavlijevic M, Cuillerier B, Wilson MJ, et al. A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3. Genomics 2002; 79:114-23. |
| Immunogen | RAET1E antibody was raised against an 18 amino acid peptide near the center of human RAET1E. The immunogen is located within amino acids 160 - 210 of RAET1E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAET1E |
|---|---|
| Alternative Names | Retinoic acid early transcript 1E NKG2D ligand 4 N2DL-4 NKG2DL42 RL-4 ULBP4 LETAL |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 29 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | RAET1E antibody is human and mouse reactive. At least four isoforms of RAET1E are known to exist; this antibody will detect all four. |
Application
Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.
Application
Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RAET1E antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RAET1E antibody is supplied in PBS containing 0.02% sodium azide. |
