Application
Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.Application
Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.Application
Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 μg/mL.| Product Name | RAIDD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1, 3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.1) Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89 |
| Immunogen | RAIDD antibody was raised against a 19 amino acid peptide near the center of human RAIDD. The immunogen is located within amino acids 90 - 140 of RAIDD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 - 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRADD |
|---|---|
| Alternative Names | RAIDD Antibody: MRT34 RAIDD Death domain-containing protein CRADD Caspase and RIP adapter with death domain |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.
Application
Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.
Application
Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 - 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RAIDD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RAIDD Antibody is supplied in PBS containing 0.02% sodium azide. |
