Application
Western blot analysis of RAIDD in MCF7 total cell lysate with RAIDD antibody at 2 μg/mL.Application
Immunohistochemistry of RAIDD in human kidney tissue with RAIDD antibody at 10 μg/mL.| Product Name | RAIDD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAIDD Antibody: MRT34, RAIDD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain |
| Product description | RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.1) Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89 |
| Immunogen | RAIDD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human RAIDD. The immunogen is located within the last 50 amino acids of RAIDD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| RAIDD antibody can be used for detection of RAIDD by Western blot at 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRADD |
|---|---|
| Alternative Names | RAIDD Antibody: MRT34 RAIDD Death domain-containing protein CRADD Caspase and RIP adapter with death domain |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | 3) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Western blot analysis of RAIDD in MCF7 total cell lysate with RAIDD antibody at 2 μg/mL.
Application
Immunohistochemistry of RAIDD in human kidney tissue with RAIDD antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | RAIDD antibody can be used for detection of RAIDD by Western blot at 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RAIDD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RAIDD Antibody is supplied in PBS containing 0.02% sodium azide. |
