Application
Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 μg/mL.Application
Immunohistochemistry of RANBP10 in human skeletal muscle tissue with RANBP10 antibody at 5 μg/mL.Application
Western blot analysis of RANBP10 in human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RANBP10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RANBP10 Antibody: The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.1) Wang D, Zaibo Li, Schoen SR, et al. A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/ERK signaling. Biochem. Biophys. Res. Comm.2004; 313:320-6. |
| Immunogen | RANBP10 antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10. The immunogen is located within amino acids 380 - 430 of RANBP10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RANBP10 |
|---|---|
| Alternative Names | RANBP10 Antibody: KIAA1464 Ran-binding protein 10 RanBP10 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%) |
Application
Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 μg/mL.
Application
Immunohistochemistry of RANBP10 in human skeletal muscle tissue with RANBP10 antibody at 5 μg/mL.
Application
Western blot analysis of RANBP10 in human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RANBP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide. |
