The product of this gene belongs to the family of
RAS-related proteins. These proteins share approximately 50% amino
acid identity with the classical RAS proteins and have numerous
structural features in common. The most striking difference between
RAP proteins and RAS proteins resides in their 61st amino acid:
glutamine in RAS is replaced by threonine in RAP proteins. The
product of this gene counteracts the mitogenic function of RAS
because it can interact with RAS GAPs and RAF in a competitive
manner. Two transcript variants encoding the same protein have been
identified for this gene.1) Liu, C., et al. Mol. Cell. Biol. 30(16):3956-3969(2010)
Immunogen
This RAP1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-170 amino acids from the C-terminal region of human RAP1A.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.