WB
Various lysates were subjected to SDS PAGE followed by western blot with RAP1A antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using RAP1A antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using RAP1A antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | RAP1A Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RAP1A protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:5000-1:50000 IHC:1:250-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | RAP1A |
|---|---|
| Gene Synonyms | RAP1 C21KG G-22K KREV1 KREV-1 SMGP21 |
| Gene Full Name | RAP1A, member of RAS oncogene family |
| Gene Summary | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Alternative Names | C21KG G 22K GTP binding protein smg p21A KREV 1 KREV1 RAP1 RAP1A Ras related protein Krev 1 Ras related protein Rap 1A SMGP21 |
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Endosome,Membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with RAP1A antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using RAP1A antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using RAP1A antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:5000-1:50000 IHC:1:250-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
