Application
Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 μg/mL.Application
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 μg/mL.Application
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 μg/mL.| Product Name | RAP80 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAP80 Antibody: RAP80, X2HRIP110, RAP80, RXRIP110, BRCA1-A complex subunit RAP80, Receptor-associated protein 80 |
| Product description | RAP80 Antibody: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.1) Yan Z, Kim YS, and Jetten AM. RAP80, a novel nuclear protein that interacts with the retinoid-related testis-associated receptor. J. Biol. Chem.2002; 277:32379-88. |
| Immunogen | RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80. The immunogen is located within amino acids 520 - 570 of RAP80. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RAP80 antibody can be used for detection of RAP80 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UIMC1 |
|---|---|
| Alternative Names | RAP80 Antibody: RAP80 X2HRIP110 RAP80 RXRIP110 BRCA1-A complex subunit RAP80 Receptor-associated protein 80 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 μg/mL.
Application
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 μg/mL.
Application
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | RAP80 antibody can be used for detection of RAP80 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RAP80 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RAP80 Antibody is supplied in PBS containing 0.02% sodium azide. |
