Application
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1μg/mLApplication
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 μg/mL.Application
Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 μg/mL.| Product Name | Raptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Raptor Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.1) Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80. |
| Immunogen | Raptor antibody was raised against a 15 amino acid synthetic peptide from the central region of human Raptor. The immunogen is located within amino acids 910 - 960 of Raptor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Rptor |
|---|---|
| Alternative Names | Raptor Antibody: Rap Raptor mKIAA1303 4932417H02Rik Regulatory-associated protein of mTOR |
| Sequence Similarities | 2) Cat. No. 17-204 - L1210 Cell Slide |
| Tissue Specificity | Raptor has multiple isoforms that may also be recognized by antibody. |
Application
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1μg/mL
Application
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 μg/mL.
Application
Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1284 - L1210 Cell Lysate |
|---|---|
| Application Notes | Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Raptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Raptor Antibody is supplied in PBS containing 0.02% sodium azide. |
