Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | Ras-GRF1 (phospho Ser916) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.1) Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. |
| Immunogen | Ras-GRF1 (phospho Ser916) antibody was raised against a peptide sequence around phosphorylation site of Serine 916 (N-E-G (p)-T P) derived from Human Ras-GRF1. |
| Modification | p-Ser916 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Rasgrf1 |
|---|---|
| Alternative Names | CDC25 GNRP GRF1 RASGRF1 |
| Molecular Weight(MW) | 145 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916. |
| Entrez Gene | 19417 |
|---|---|
| Protein Accession | NP_002882.3 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left) or the same antibody preincubated with blocking peptide (right).| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
