Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)| Product Name | RASGRF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.1) Iguchi,T., Int. J. Oncol. 31 (2), 285-291 (2007) |
| Immunogen | This RASGRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 907-936 amino acids from human RASGRF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RASGRF1 |
|---|---|
| Alternative Names | Ras-specific guanine nucleotide-releasing factor 1 Ras-GRF1 Guanine nucleotide-releasing protein GNRP Ras-specific nucleotide exchange factor CDC25 RASGRF1 CDC25 GNRP GRF1 |
| Molecular Weight(MW) | 145 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
