Application
RASGRP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | RASGRP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This RASGRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-521 amino acids from the Central region of human RASGRP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RASGRP1 |
|---|---|
| Alternative Names | RAS guanyl-releasing protein 1 Calcium and DAG-regulated guanine nucleotide exchange factor II CalDAG-GEFII Ras guanyl-releasing protein RASGRP1 RASGRP |
| Molecular Weight(MW) | 90 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
RASGRP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
