Application
Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 μg/mL.Application
Immunohistochemistry of RBBP8 in rat spleen tissue with RBBP8 antibody at 5 μg/mL.Application
Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RBBP8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RBBP8 Antibody: RBBP8, also known as CtBP (carboxy-terminal binding protein) interacting protein (CTIP), was characterized for its role in transcription as a cofactor for the transcriptional repressor CtBP, and also as a binding partner for other proteins including the cell cycle regulators retinoblastoma protein (Rb) and breast cancer 1 (BRCA1). It is ubiquitously expressed and localizes to the nucleus. RBBP8 is thought to modulate the functions in cell proliferation, transcriptional regulation and DNA repair. RBBP8 also plays a central role in the cell cycle checkpoint response to DNA double-stranded breaks (DSBs), with new evidence demonstrating that it controls the choice of DSB repair pathway.1) Yu X, Wu LC, Bowcock AM, et al. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression. J. Biol. Chem.1998; 273:25388-92. |
| Immunogen | RBBP8 antibody was raised against a 14 amino acid synthetic peptide near the center of human RBBP8. The immunogen is located within amino acids 430 - 480 of RBBP8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBBP8 |
|---|---|
| Alternative Names | RBBP8 Antibody: RIM COM1 CTIP JWDS SAE2 SCKL2 DNA endonuclease RBBP8 CtBP-interacting protein CtIP |
Application
Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 μg/mL.
Application
Immunohistochemistry of RBBP8 in rat spleen tissue with RBBP8 antibody at 5 μg/mL.
Application
Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RBBP8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RBBP8 Antibody is supplied in PBS containing 0.02% sodium azide. |
