Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded brain tissue reacted with RBM14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of RBM14 Antibody in Jurkat cell line lysates (35ug/lane)| Product Name | RBM14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNA-binding protein 14, Paraspeckle protein 2, PSP2, RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, Synaptotagmin-interacting protein, SYT-interacting protein, RBM14, SIP |
| Product description | Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.1) Andersen,J.S., et.al., Curr. Biol. 12 (1), 1-11 (2002) |
| Immunogen | This RBM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-223 amino acids from the Central region of human RBM14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | RBM14 |
|---|---|
| Alternative Names | RNA-binding protein 14 Paraspeckle protein 2 PSP2 RNA-binding motif protein 14 RRM-containing coactivator activator/modulator Synaptotagmin-interacting protein SYT-interacting protein RBM14 SIP |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded brain tissue reacted with RBM14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of RBM14 Antibody in Jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
