WB
Western blot analysis of various lysates using RBM14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using RBM14 Rabbit pAb at dilution of 1:300 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cellsusing RBM14 Rabbit pAb at dilutionof 1:300 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.| Product Name | RBM14 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBM14 |
|---|---|
| Gene Synonyms | SIP COAA PSP2 SYTIP1 TMEM137 |
| Gene Full Name | RNA binding motif protein 14 |
| Gene Summary | This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | SIP COAA PSP2 SYTIP1 TMEM137 RBM14 |
| Molecular Weight(MW) | 69kDa(Observed band size:75kDa) |
| Cellular Localization | Nucleus, nucleolus. |
WB
Western blot analysis of various lysates using RBM14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using RBM14 Rabbit pAb at dilution of 1:300 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cellsusing RBM14 Rabbit pAb at dilutionof 1:300 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
