Application
Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 μg/mL.Application
Immunofluorescence of RBM35A in human colon tissue cells with RBM35A antibody at 20 μg/mL.Application
Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | RBM35A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBM35A Antibody: RBM35A, RMB35A, RBM35A, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A |
| Product description | RBM35A Antibody: RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.1) Warzecha CC, Jiang P, Amirikian K, et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J. 2010; 29:3286-300. |
| Immunogen | RBM35A antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A. The immunogen is located within amino acids 30 - 80 of RBM35A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESRP1 |
|---|---|
| Alternative Names | RBM35A Antibody: RBM35A RMB35A RBM35A Epithelial splicing regulatory protein 1 RNA-binding motif protein 35A |
| Tissue Specificity | RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five. |
| Entrez Gene | 54845 |
|---|---|
| Protein Accession | NP_001030087 |
Application
Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 μg/mL.
Application
Immunofluorescence of RBM35A in human colon tissue cells with RBM35A antibody at 20 μg/mL.
Application
Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1472 - Rat Colon Tissue Lysate |
|---|---|
| Application Notes | RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RBM35A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RBM35A Antibody is supplied in PBS containing 0.02% sodium azide. |
