Application
Western Blot at 1:2000 dilution Lane 1: mouse eyeball lysate Lane 2: mouse lung lysate Lysates/proteins at 20 ug per lane.| Product Name | RBP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Retinol-binding protein 3, Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3 |
| Product description | Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.1) Garcia-Ramirez, M., et al. Diabetologia 52(12):2633-2641(2009) |
| Immunogen | This RBP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the Central region of human RBP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBP3 |
|---|---|
| Alternative Names | Retinol-binding protein 3 Interphotoreceptor retinoid-binding protein IRBP Interstitial retinol-binding protein RBP3 |
| Molecular Weight(MW) | 135 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: mouse eyeball lysate Lane 2: mouse lung lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
