Application
Immunofluorescence of RC3H1 in human small intestine tissue with RC3H1 antibody at 20 μg/ml.Application
Immunohistochemistry of RC3H1 in human small intestine tissue with RC3H1 antibody at 5 μg/ml.Application
Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 antibody at 1 μg/mL| Product Name | RC3H1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RC3H1 Antibody: RNF198, ROQUIN, KIAA2025, RNF198, Roquin-1, RING finger and C3H zinc finger protein 1, Roquin |
| Product description | RC3H1 Antibody: The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.1) Vinuesa CG, Cook MC, Angelucci C, et al. A RING-type ubiquitin ligase family member required to repress follicular helper T cells and autoimmunity. Nature 2005; 435:452-8. |
| Immunogen | Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1. The immunogen is located within amino acids 1040 - 1090 of RC3H1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RC3H1 |
|---|---|
| Alternative Names | RC3H1 Antibody: RNF198 ROQUIN KIAA2025 RNF198 Roquin-1 RING finger and C3H zinc finger protein 1 Roquin |
| Molecular Weight(MW) | 125 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms. |
Application
Immunofluorescence of RC3H1 in human small intestine tissue with RC3H1 antibody at 20 μg/ml.
Application
Immunohistochemistry of RC3H1 in human small intestine tissue with RC3H1 antibody at 5 μg/ml.
Application
Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 antibody at 1 μg/mL| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RC3H1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RC3H1 Antibody is supplied in PBS containing 0.02% sodium azide. |
