Application
Immunohistochemistry of RCAN2 in mouse brain tissue with RCAN2 antibody at 2.5 μg/mL.Application
Western blot analysis of RCAN2 in 3T3 cell lysate with RCAN2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of RCAN2 in mouse brain tissue with RCAN2 antibody at 20 μg/mL.| Product Name | RCAN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RCAN2 Antibody: Csp2, MCIP2, ZAKI-4, Dscr1l1, Zaki4, Calcipressin-2, Calcineurin inhibitory protein ZAKI-4 |
| Product description | RCAN2 Antibody: Regulator of calcineurin 2 (RCAN2), also known as ZAKI4 and DSCR1L1, is expressed as two isoforms differing at their N-terminus. The longer of the two (isoform 1) is expressed exclusively in the brain, while isoform 2 is ubiquitously expressed, with highest expression in brain, heart, and muscle. Both isoforms bind to the catalytic subunit of calcineurin, a Ca++-dependent protein phosphatase involved in several neuronal functions, though their C-terminal region and inhibit calcineurin's activity. Unlike isoform 1 of RCAN2, the expression of the second isoform is not induced by the thyroid hormone T3. RCAN2 is a member of a family of three endogenous calcineurin regulators that are located near the minimal supernumerary fragment of chromosome 21 in individuals with Down syndrome, suggesting that they play a role in this syndrome. Multiple isoforms of RCAN2 are known to exist.1) Miyazaki T, Kanou Y, Murata Y, et al. Molecular cloning of a novel thyroid hormone-responsive gene, ZAKI-4, in human skin fibroblasts. J. Biol. Chem.1996; 271:14567-71. |
| Immunogen | RCAN2 antibody was raised against a 14 amino acid synthetic peptide near the center of human RCAN2. The immunogen is located within amino acids 70 - 120 of RCAN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RCAN2 antibody can be used for detection of RCAN2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Rcan2 |
|---|---|
| Alternative Names | RCAN2 Antibody: Csp2 MCIP2 ZAKI-4 Dscr1l1 Zaki4 Calcipressin-2 Calcineurin inhibitory protein ZAKI-4 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Immunohistochemistry of RCAN2 in mouse brain tissue with RCAN2 antibody at 2.5 μg/mL.
Application
Western blot analysis of RCAN2 in 3T3 cell lysate with RCAN2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of RCAN2 in mouse brain tissue with RCAN2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | RCAN2 antibody can be used for detection of RCAN2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RCAN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RCAN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
