Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RCE1 using rabbit polyclonal RCE1 Antibody (W57) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCE1 gene.Application
RCE1 Antibody (N-term W57) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RCE1 Antibody (N-term W57) for immunohistochemistry.| Product Name | RCE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAAX prenyl protease 2, 3422-, Farnesylated proteins-converting enzyme 2, FACE-2, Prenyl protein-specific endoprotease 2, RCE1 homolog, hRCE1, RCE1, FACE2, RCE1A, RCE1B |
| Product description | RCE1 is an integral membrane protein which is classified as a member of the metalloproteinase family. This enzyme is thought to function in the maintenance and processing of CAAX-type prenylated proteins.1) Maske, C.P., et al., J. Cell Biol. 162(7):1223-1232 (2003). |
| Immunogen | This RCE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human RCE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RCE1 |
|---|---|
| Alternative Names | CAAX prenyl protease 2 3422- Farnesylated proteins-converting enzyme 2 FACE-2 Prenyl protein-specific endoprotease 2 RCE1 homolog hRCE1 RCE1 FACE2 RCE1A RCE1B |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RCE1 using rabbit polyclonal RCE1 Antibody (W57) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCE1 gene.
Application
RCE1 Antibody (N-term W57) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RCE1 Antibody (N-term W57) for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
