Application
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody overnight. The membrane was incubated with donkey anti-rabbit antibody for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data| Product Name | RENT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Regulator of nonsense transcripts 1, 364-, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, UPF1, KIAA0221, RENT1 |
| Product description | RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.1) Ohnishi, T., et al., Mol. Cell 12(5):1187-1200 (2003). Lykke-Andersen, J., Mol. Cell. Biol. 22(23):8114-8121 (2002). Carastro, L.M., et al., Nucleic Acids Res. 30(10):2232-2243 (2002). Mendell, J.T., et al., Science 298(5592):419-422 (2002). Serin, G., et al., Mol. Cell. Biol. 21(1):209-223 (2001). |
| Immunogen | This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the Central region of human RENT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | UPF1 |
|---|---|
| Alternative Names | Regulator of nonsense transcripts 1 364- ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 NORF1 Up-frameshift suppressor 1 homolog hUpf1 UPF1 KIAA0221 RENT1 |
| Molecular Weight(MW) | 124 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody overnight. The membrane was incubated with donkey anti-rabbit antibody for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
