Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. REPIN1 binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. REPIN1 facilitates DNA bending.1) Kim, M.Y., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (35), 13074-13079.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human REPIN1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
REPIN1 antibody can be used for detection of REPIN1 by ELISA at 1:312500. REPIN1 antibody can be used for detection of REPIN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Heart lysate at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Application Notes
REPIN1 antibody can be used for detection of REPIN1 by ELISA at 1:312500. REPIN1 antibody can be used for detection of REPIN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store REPIN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.