Application
Immunohistochemistry of REQUIEM in human kidney tissue with REQUIEM antibody at 5 μg/mL.Application
Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 μg/mL.Application
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 μg/mL.| Product Name | REQUIEM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | REQUIEM Antibody: REQ, UBID4, ubi-d4, BAF45D, REQ, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein |
| Product description | REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.1) Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9. |
| Immunogen | REQUIEM antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Requiem. The immunogen is located within the last 50 amino acids of REQUIEM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPF2 |
|---|---|
| Alternative Names | REQUIEM Antibody: REQ UBID4 ubi-d4 BAF45D REQ Zinc finger protein ubi-d4 Apoptosis response zinc finger protein |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | Requiem antibody is predicted to not cross-react with other DPF protein family members. |
Application
Immunohistochemistry of REQUIEM in human kidney tissue with REQUIEM antibody at 5 μg/mL.
Application
Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 μg/mL.
Application
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | REQUIEM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | REQUIEM Antibody is supplied in PBS containing 0.02% sodium azide. |
