Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.Application
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.| Product Name | Resistin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adipocyte Secreted Factor, ADSF, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, Resistin, Adipose tissue-specific secretory factor, XCP1, RETN1 |
| Product description | Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. |
| Immunogen | Resistin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL. Western Blot: To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RETN |
|---|---|
| Alternative Names | Adipocyte Secreted Factor ADSF FIZZ3 HXCP1 RSTN UNQ407/PRO1199 Resistin Adipose tissue-specific secretory factor XCP1 RETN1 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
Application
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL. Western Blot: To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Resistin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
