Application
Flow cytometric analysis of MCF-7 cells using RET Antibody (N-term Q28) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of hRET-G28 in MCF7 cell line lysates (35ug/lane)| Product Name | RET Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51 |
| Product description | RET, a member of the cadherin superfamily, is one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This protein plays a crucial role in neural crest development, and the gene can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations i are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.1) Da Silva, A.M., et al., J. Clin. Endocrinol. Metab. 88(11):5438-5443 (2003). |
| Immunogen | This RET antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-44 amino acids from the N-terminal region of human RET. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | RET |
|---|---|
| Alternative Names | Proto-oncogene tyrosine-protein kinase receptor Ret Cadherin family member 12 Proto-oncogene c-Ret Soluble RET kinase fragment Extracellular cell-membrane anchored RET cadherin 120 kDa fragment RET CDHF12 CDHR16 PTC RET51 |
| Molecular Weight(MW) | 124 kDa |
Application
Flow cytometric analysis of MCF-7 cells using RET Antibody (N-term Q28) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of hRET-G28 in MCF7 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
